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Antibodies bound to the blocking peptide no longer bind for the epitope to the target protein. By comparing the staining from the blocked antibody vs . the antibody by yourself, one can see which staining is certain.
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This guide outlines additional attainable optimization of cellular and immunoassay parameters to guarantee the very best final results are...
This manual outlines further probable optimization of cellular and immunoassay parameters to make certain the best possible outcomes are...
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The antibody is from purified rabbit serum by affinity purification via sequential chromatography on phospho-peptide and non-phospho-peptide affinity columns.
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The measurement of protein phosphorylation is a useful tool for measuring the modulation of receptor activation by both antibodies...
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Blocking peptides are peptides that bind particularly for the target antibody and block antibody binding. These peptide ordinarily consists of the epitope regarded from the antibody.
Goal Information and facts Cyclin E belongs to your highly conserved cyclin relatives, whose users are characterized by a remarkable periodicity in protein abundance from the cell cycle. Cyclins perform as regulators of CDK kinases. Different cyclins show unique expression and degradation patterns which add on the temporal coordination of each mitotic party. Cyclin E varieties a complex with and capabilities like a regulatory subunit of CDK2, whose activity is necessary for cell cycle G1/S changeover. This protein accumulates with the G1-S phase boundary and is particularly degraded as cells progress as a result of S section.
Overexpression of the gene continues to be observed in many tumors, which leads to chromosome instability, and therefore might add to tumorigenesis. This protein was identified to affiliate with, and become associated with, the phosphorylation of NPAT protein (nuclear protein mapped into the ATM locus), which participates in cell-cycle controlled histone gene expression and performs a crucial purpose in selling mobile-cycle development in the absence of pRB. Two alternatively spliced transcript variants of the gene, which encode distinct isoforms, are described. Two added splice variants ended up reported but in depth click here nucleotide sequence facts is not really yet accessible.
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